Deputy Marshal Chambers was shot and killed by a man he had encountered the previous night. The man walked up to Deputy Chambers at midnight, on Main Street in front of the Central Saloon on Madison Avenue, with a gun concealed in a newspaper and shot Chambers four times. Deputy Chambers staggered across the street and fell in front of the Bank of Aurora. His sidearm, which he had attempted to draw in his defense fell to the ground and discharged, the bullet grazing the calf of the leg of Marshal William Connor who was some distance away.  The suspect, Eugene Jarrett, 28, ran but was arrested shortly thereafter by Marshal Conner and placed into the county jail at Mt. Vernon. Jarrett and Deputy Chambers had quarreled the night before when a crowd of Jarrett's friends were being dispersed by Deputy Chambers. Eugene Jarrett was the son of local prominent mill owner John Jarrett of Aurora. In November 1919, Jarrett was tried in Mt. Vernon and acquitted of a 1st Degree Murder charge in the killing in what was described as an unexpected ruling.

Chambers was survived by his wife and five children. Interred: Maple Park Cemetery, Aurora, MO.